This article explains the installation of the HEPA filter kit (Part number 601-3149-001) for the FinePointe WBP system. Two configurations are covered. The first configuration is for use in a BSL3/4 environment using a fume hood and the bias flow set to push mode. The second configuration is the standard set up with no fume hood using pull bias flow mode.

HEPA filter kit parts required for use with a FinePointe WBP system

• TSI HEPA capsule filter assembly (Fig 1) - This part has 3/8" NPT to 5/16" fittings mounted on each end for connecting to 5/16" OD tubing. The assembly is leak tested at DSI to insure proper performance.
• 10 feet of 5/16" OD Tygon tubing - This tubing connects the HEPA filter to the WBP controller and a WBP chamber. This is the same type of tubing we perform the HEPA filter capsule leak test with to insure a proper connection. The standard bias flow tubing is not rigid enough for use with the HEPA filter.
• The other parts included in the kit are not required for use with the WBP system. 

Fig 1 - TSI HEPA capsule filter assembly:

Connecting the HEPA filter in a BSL 3/4 laboratory with a fume hood

1.  Remove the standard WBP bias flow filter/tubing set from each site and perform these tasks:
   1. Remove the white plastic luer fitting that connects to the WBP chamber from the tubing. Save the luer fitting for use with the tubing included with the HEPA filter. 
   2. Remove the clear plastic filter that connects to the bias flow port on the WBP controller from the tubing. You may need to cut a small slit in the old tubing where it connects to this filter to remove it as It can be very tight. Save the plastic bias flow filter for use with the tubing included with the HEPA filter.
2. Estimate length the entire HEPA/tubing assembly needs to be to reach from the WBP controller bias flow port to the WBP chamber located inside the fume hood. The maximum length will be a little over 10 feet with the new tubing. You need to consider the length of the HEPA filter in this estimate. The idea is to have the HEPA filter 12-18 inches from the bias flow port on the WBP controller so it can be placed on the same surface as the controller. The rest of the tubing will extend from the other end of the HEPA filter to the WBP chamber under the fume hood. Use the diagram following this section as a reference (Fig 2). 
3. After estimating the total length, cut the section of tubing you need to connect the small plastic bias flow filter you saved in step 1.2 to the HEPA filter. This should be about 12-18 inches as long. Make sure you cut the tips of the tubing straight. 
4. Next, cut the section of tubing you need to reach from the HEPA filter to the chamber in the fume hood. This could be another 8 feet depending on how far away the chamber is. It's better to have a few feet of extra length in case you have to move things around. Make sure you cut the tips of the tubing straight.
5. Connect one end of the 12-18" section of tubing to the plastic bias flow filter and the other end to the inlet side of the HEPA filter. The black arrow on the HEPA filter should be pointed towards the WBP chamber to function with the push bias flow. The tubing should be firmly pushed into the fitting on the HEPA filter until it will go no further. (Fig 2)
6. Connect the longer length of tubing to outlet end of the HEPA filter. The tubing should be firmly pushed into the fitting on the HEPA filter until it will go no further. Connect the other end of the tubing to the white luer fitting (saved in step 1.1) that connects to the WBP chamber bias flow port. 
7. Next, connect the assembly to the system by plugging the end with the plastic bias flow filter into the appropriate bias flow port on the WBP controller and the other end with the white luer fitting into the WBP chamber bias flow port. 
8. INSURE THAT THE BIAS FLOW IS ALWAY USED IN PUSH MODE WHEN THE SYSTEM IS USED IN A BSL3 OR BSL4 LABORATORY!!!

Fig 2 - HEPA Filter BSL 3/4 configuration:

Connecting the HEPA filter using the standard bias flow pull mode with no fume hood

This configuration is the same as the BSL 3/4 configuration above except for the following:

1. Orient the HEPA filter so the black arrow is pointed towards the WBP controller, not the WBP chamber.
2. The WBP chamber is not placed under a fume hood.
3. The bias flow should be set to pull mode. (Push mode is possible if you turn the HEPA filter around so the flow arrow points toward the chamber)
4. Since the chamber is usually placed closer to the controller in this setup, the section of tubing that connects the HEPA filter to the chamber is usually shorter. 
5. See Fig 3 below for details. 

Fig 3 - HEPA Filter standard configuration: