The article covers known health issues after exposure to smoke.
Mucus in the Eyes
Mucus in the eyes can be seen when the bias flow is not high enough and/or when the exposure period is too long. For example, if you are seeing mucus in the eyes after 30 minutes exposure period with the flow set to 2 LPM, double the rate to 4 LPM over the 30 minutes exposure period is likely to resolve this issue.
Maximize Flow Rate
Turn the flow unit up to 5 LPM and remove the caps from the 6 ports on both ends of the chamber. To insure an even amount of smoke enters the chamber, you can split the tubing to connect to all four input ports on the top of the chamber. Enough tubing and and fittings are included with the system to configure it this way (image below).
Significant Reduction in Body Temperature
Nicotine disrupts the linear relationship between heart rate and body temperature (Sack et al., 2005). Please see the following publications for more information:
Vanderstocken, G., Marrow, J. P., Allwood, M. A., Stampfli, M. R., & Simpson, J. A. (2020). Disruption of Physiological Rhythms Persist Following Cessation of Cigarette Smoke Exposure in Mice. Frontiers in Physiology, 11(October), 1–13. https://doi.org/10.3389/fphys.2020.501383